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Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM)
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DOI：10.1364/OE.15.006420.png)
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摘要
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Advances in the life sciences rely on the ability to observe dynamic processes in live systems and in environments that mimic in-vivo situations. Therefore, new methodological developments have to provide environments that resemble physiologically and clinically relevant conditions as closely as possible. In this work, plasma-induced laser nanosurgery for three-dimensional sample manipulation and sample perturbation is combined with optically sectioning light-sheet based fluorescence microscopy (SPIM) and applied to three-dimensional biological model systems. This means: a) working with a biological system that is not confined to essentially two dimensions like cell cultures on cover glasses, b) gaining intrinsic optical sectioning capabilities by an efficient three-dimensional fluorescence imaging system, and c) using arbitrarily-shaped three-dimensional ablation-patterns by a plasma-induced laser ablation system that prevent damage to surrounding tissues. Spatial levels in our biological applications range from sub-microns during delicate ablation of single microtubules over the confined disruption of cell membranes in an MDCK-cyst to the macroscopic cutting of a millimeter-sized Zebrafish caudal fin with arbitrary three-dimensional ablation patterns. Dynamic processes like laser-induced hemocyte migration can be studied with our SPIM-microscalpel in intact, live embryos. (C) 2007 Optical Society of America
Keyword:
IN-VIVO
BIOLOGICAL TISSUES
DROSOPHILA EMBRYOS
DORSAL CLOSURE
CELL
FORCES
MORPHOGENESIS
MICROTUBULES
NANOSURGERY
ABLATION
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
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High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy.png)
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NATURE METHODSIF32.1
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