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Time-lapse imaging beyond the diffraction limit
DOI:10.1016/j.ymeth.2018.07.004.png)
摘要
En 中文
The zebrafish, with its rapid external development, optical transparency, and the relative ease with which transgenic lines can be created, is rapidly becoming the model of choice for examining developmental processes via time-lapse microscopy. The recent proliferation of techniques for super-resolution imaging now allows for an unprecedented view of embryonic development at high spatial and temporal resolution in live tissues. This review examines both the theoretical basis and practical application of a number of established and emerging super-resolution microscopy techniques, focusing on their application in time-lapse imaging of live zebrafish embryos.
Keyword:
STRUCTURED ILLUMINATION MICROSCOPY
FLUORESCENCE MICROSCOPY
PLANE ILLUMINATION
RESOLUTION LIMIT
LIVE
NANOSCOPY
CELLS
EMISSION
DYNAMICS
EMBRYOS
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期刊
IF:
4.3
论文数:
4.9K
被引数:
2.4W
机构
引用论文
Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison
SCIENTIFIC REPORTS
IF3.9

