Top-Down Proteomics: Why and When?

Philipp T. Kaulich

doi:10.1002/pmic.202400338

2025-04-27

Philipp T. Kaulich

Andreas Tholey *

DOI：10.1002/pmic.202400338

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摘要

En 中文

多种生物学过程在转录和翻译的所有水平上均可导致大量不同蛋白质种类（即蛋白质异构体）的形成，这些种类数量远超基因组编码的序列。由于以这种方式形成的蛋白质分子数量庞大且具有广泛的物理化学性质差异，蛋白质异构体是所有生物学过程的功能驱动者，这促使需要强大的分析方法来解读这一生命语言。尽管自下而上蛋白质组学已成为最广泛应用的方法，提供了高灵敏度、分析深度和通量等特征，但在识别、定量和表征蛋白质异构体方面仍存在局限性。特别是其主要瓶颈在于将肽段水平信息分配给原始蛋白质异构体。相比之下，自上而下蛋白质组学（TDP）直接针对完整蛋白质异构体的分析。尽管面临多项技术挑战，TDP社群已建立多种方案，允许在任意蛋白质组学实验室中轻松实施。在本观点中，我们比较了这两种方法，论证了嵌入TDP实验的价值，并展示了TDP可在其中成功实施以执行整合多级蛋白质异构体组学的科研领域。

Keyword:

CE-MS

LC-MS

posttranslational modification

proteoform

proteoformics

quantification

splicing

期刊

Proteomics

IF:

3.9

论文数:

7.6K

被引数:

1.1W

机构

christian-albrechts-universität zu kiel

学者数:

论文数: 52

被引数: 0

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