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UCsim2: two-dimensionally structured illumination microscopy using UC2
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DOI：10.1098/rsta.2020.0148.png)
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摘要
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State-of-the-art microscopy techniques enable the imaging of sub-diffraction barrier biological structures at the price of high costs or a lack of transparency. We try to reduce some of these barriers by presenting a super-resolution upgrade to our recently presented open-source optical toolbox UC2. Our new injection moulded parts allow larger builds with higher precision. The 4x lower manufacturing tolerance compared to three-dimensional printing makes assemblies more reproducible. By adding consumer-grade available open-source hardware such as digital mirror devices and laser projectors, we demonstrate a compact three-dimensional multimodal setup that combines image scanning microscopy and structured illumination microscopy. We demonstrate a gain in resolution and optical sectioning using the two different modes compared to the widefield limit by imaging Alexa Fluor (R) 647- and Silicon Rhodamine-stained HeLa cells. We compare different objective lenses and by sharing the designs and manuals of our setup, we make super-resolution imaging available to everyone. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.
Keyword:
modular microscopy
education
SIM
ISM
UC2
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3.7
论文数:
7.8K
被引数:
2.8W
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OPTICS LETTERSIF3.3
PREAI
分享收藏A versatile and customizable low-cost 3D-printed open standard for microscopic imaging用于显微成像的多功能且可定制的低成本3d打印开放标准NATURE COMMUNICATIONS
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NATURE COMMUNICATIONSIF15.7
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