.png){kind=icon}
返回
Yeast surface display for screening combinatorial polypeptide libraries
.png){kind=icon}
PRE
AI
DOI：10.1038/nbt0697-553.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity. C-terminal fusion to the Aga2p mating adhesion receptor of Saccharomyces cerevisiae has been used for the selection of scFv antibody fragments with threefold decreased antigen dissociation rate from a randomly mutated library. A eukaryotic host should alleviate expression biases present in bacterially propagated combinatorial libraries. Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands.
Keyword:
antibody engineering
combinatorial library
surface display
affinity maturation
scFv
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
41.7
论文数:
1.2W
被引数:
10.1W
机构
暂无机构信息
引用论文
Large‐scale expansion of rat CD4+ CD25+ Treg cells in the absence of T‐cell receptor stimulation.png)
Immunology
OAAI
ImmunologyIF0
OAAI分享收藏Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display libraryNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines在微生物表面展示异源蛋白质: 从组合文库的筛选到活的重组疫苗NATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏
查看更多内容