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A parallel microfluidic flow cytometer for high-content screening
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DOI：10.1038/NMETH.1595.png)
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Abstract
En 中文
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMCMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NF kappa B-EGFP reporter.
Keywords:
NF-KAPPA-B
MICROSCOPY
ALPHA
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