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A STED Microscope Designed for Routine Biomedical Applications
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DOI：10.2528/PIER14042708.png)
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Abstract
En 中文
We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.
Keywords:
DEPLETION FLUORESCENCE MICROSCOPY
DIFFRACTION RESOLUTION LIMIT
STIMULATED-EMISSION
NANOSCOPY
BREAKING
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