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Annotating genomes with massive-scale RNA sequencing
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DOI：10.1186/gb-2008-9-12-r175.png)
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Abstract
En 中文
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
Keywords:
DNA-SEQUENCES
GENERATION
DISCOVERY
MAPS
BREAKPOINTS
PROGRAM
BLAST
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