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Artifact-free high-density localization microscopy analysis

delete2018-07-30
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PRE
AI
R
Richard J. Marsh
K
Karin Pfisterer
P
Pauline M. Bennett
L
L Hirvonen
M
Mathias Gautel
G
Gareth E. Jones
S
Susan Cox *
DOI:10.1038/s41592-018-0072-5delete
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Abstract

Abstract

En 中文
High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling highspeed, artifact-free super-resolution imaging of live cells.
Keywords:
IMMUNOELECTRON MICROSCOPY
TITIN
STORM
NANOSCOPY
MUSCLE
IMAGEJ
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Journal

Nature Methods cover
Nature Methods
IF:
32.1
Papers:
7.2K
Citations:
12.7W

Organization

U
university of london
Scholars:
21.5W
Papers: 19.7W
Citations: 305