.png){kind=icon}
Return
Combinatorial Fc modifications for complementary antibody functionality
.png){kind=icon}
OA
AI
DOI：10.1080/19420862.2025.2465391.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Therapeutic monoclonal antibodies (mAbs) can be functionally enhanced via Fc engineering. To determine whether pairs of mAbs with different Fc modifications can be combined for functional complementarity, we investigated the in vitro activity of two HIV-1 mAb libraries, each equipped with 60 engineered Fc variants. Our findings demonstrate that the impact of Fc engineering on Fc functionality is dependent on the specific Fab clone. Notably, combinations of Fc variants of the same Fab specificity exhibited limited enhancement in functional breadth compared to combinations involving two distinct Fabs. This suggests that the strategic selection of complementary Fc modifications can enhance both functional activity and breadth. Furthermore, while some combinations of Fc variants displayed additive functional effects, others were detrimental, suggesting that the functional outcome of Fc mutations is not easily predicted. Collectively, these results provide preliminary evidence supporting the potential of complementary Fc modifications in mAb combinations. Future studies will be essential to identify the optimal Fc modifications that maximize in vivo efficacy.
Keywords:
Broadly functional antibodies
complimentary combinations
Fc-modifications
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
7.3
Papers:
1.8K
Citations:
7.2K
Organization
Cited Papers
A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samplesOAAI
OAAIShareSaveDissecting strategies to tune the therapeutic potential of SARS-CoV-2-specific monoclonal antibody CR3022.png)
JCI INSIGHT
OAAI
JCI INSIGHTIF6.1
OAAIShareSaveShareSaveShareSaveShareSaveShareSave
View more