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Endoscopic diffraction phase microscopy
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DOI：10.1364/OL.43.003373.png)
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Abstract
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In this Letter, we present, to our knowledge, the first endoscopic diffraction phase microscopy (eDPM) system. This instrument consists of a gradient-index-lens-based endoscope probe followed by a DPM module, which enables single-shot phase imaging at a single-cell-level resolution. Using the phase information provided by eDPM, we show that the geometric aberrations associated with the endoscope can be reduced by digitally applying a spectral phase filter to the raw data. The filter function is a linear combination of polynomials with weighting optimized to improve resolution. We validate the principle of the proposed method using reflective semiconductor samples and blood cells. This research extends the current scope of quantitative phase imaging applications, and proves its potential for future in vivo studies. (C) 2018 Optical Society of America
Keywords:
DIGITAL HOLOGRAPHIC MICROSCOPY
QUANTITATIVE PHASE
WHITE-LIGHT
IN-VIVO
TOMOGRAPHY
CELLS
ILLUMINATION
DYNAMICS
TISSUE
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