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Engineering and application of a recombinant Aspergillus niger CECT 2907 tannase to enhance the antioxidant activity of black tea
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DOI：10.1002/jsfa.70834.png)
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Abstract
En 中文
Tannases (tannin acyl hydrolases, EC 3.1.1.20) catalyze the hydrolysis of galloylated polyphenols, releasing gallic acid and other bioactive compounds with antioxidant properties. These enzymes have a wide range of applications in the food and beverage industry, particularly for improving tea quality. In this study, a tannase gene from Aspergillus niger CECT 2907 was cloned and heterologously expressed in Komagataella phaffii. Two recombinant variants were generated: a double-chain enzyme (AnTanDC) and a single-chain variant (AnTanSC) obtained by mutating two Kex2 protease cleavage sites. Both enzymes were biochemically characterized, and the potential of AnTanSC to enhance the antioxidant properties of black tea was evaluated.
Keywords:
antioxidant activity
Aspergillus awamori NRRL 3112
enzyme engineering
gallic acid
Pichia pastoris
tannin acyl hydrolase
tea polyphenols
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IF:
3.5
Papers:
1.4W
Citations:
4.1W
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