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Enhancing diffractive multi-plane microscopy using colored illumination
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DOI：10.1364/OE.21.011150.png)
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Abstract
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We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes. We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy. (C) 2013 Optical Society of America
Keywords:
POINT-SPREAD FUNCTION
FLUORESCENCE MICROSCOPY
HOLOGRAPHIC MICROSCOPY
LIVING CELLS
3 DIMENSIONS
TRACKING
LOCALIZATION
EFFICIENCY
LIGHT
LIMIT
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