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Enhancing gel properties of channel catfish (Ictalurus punctatus) myofibrillar protein by high-voltage electrostatic field: The role of protein oxidation, nitration, and structural changes
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DOI：10.1016/j.ifset.2025.104387.png)
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Abstract
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• HVEF induced ROS/RNS generation, causing MPs oxidation and nitration. • Site-specific oxidative/nitrative modifications in MHC were identified via proteomics. • HVEF disrupted secondary structure, reduced hydrophobicity, and promoted crosslinking. • Protein modifications enhanced surface electronegativity, rheology, and gel strength. • Optimal functional properties were achieved after 20 min of HVEF treatment.
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