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Gene expression profiling in single cells within tissue
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DOI：10.1038/NMETH786.png)
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Abstract
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We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
Keywords:
PROSTATE-CANCER
BREAST-CANCER
ARRAYS
ASSAY
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