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High-throughput single-cell omics using semipermeable capsules
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DOI：10.1126/science.ady7227.png)
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Abstract
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Profiling single cells across multiple modalities requires complex, multistep molecular biology workflows that are difficult to scale. Two studies developed semipermeable capsule (SPC) technology in which microscopic liquid vessels are enclosed by a thin, biodegradable shell that efficiently retains nucleic acids of encapsulated cells while remaining permeable to enzymes, oligonucleotides, and other reagents. Mazelis et al. implemented a barcoding method to enable single-capsule readouts and then used these barcodes to study how gene expression patterns persist as cells divide. Baronas et al. implemented a single-cell RNA-sequencing approach that enables accurate and comprehensive capture of diverse cell types in circulating cells of acute myeloid leukemia patients, uncovering distinct leukemic cell phenotypes. SPCs offer a versatile and scalable platform for single-cell omics assays, including RNA and genome sequencing, nucleic acid–guided cell sorting, and long-term cell cultivation. —Di Jiang
Keywords:
single-cell omics
semipermeable capsules
barcoding
RNA sequencing
cell phenotypes
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