.png){kind=icon}
Return
Highly sensitive and specific microRNA expression profiling using BeadArray technology
.png){kind=icon}
OA
AI
DOI：10.1093/nar/gkn387.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
We have developed a highly sensitive, specific and reproducible method for microRNA (miRNA) expression profiling, using the BeadArray technology. This method incorporates an enzyme-assisted specificity step, a solid-phase primer extension to distinguish between members of miRNA families. In addition, a universal PCR is used to amplify all targets prior to array hybridization. Currently, assay probes are designed to simultaneously analyse 735 well-annotated human miRNAs. Using this method, highly reproducible miRNA expression profiles were generated with 100-200 ng total RNA input. Furthermore, very similar expression profiles were obtained with total RNA and enriched small RNA species (R-2 >= 0.97). The method has a 3.5-4 log (10(5)-10(9) molecules) dynamic range and is able to detect 1.2- to 1.3-fold-differences between samples. Expression profiles generated by this method are highly comparable to those obtained with RTPCR (R-2 = 0.850.90) and direct sequencing (R = 0.870.89). This method, in conjunction with the 96-sample array matrix should prove useful for high-throughput expression profiling of miRNAs in large numbers of tissue samples.
Keywords:
MAMMALIAN MICRORNAS
IDENTIFICATION
SIGNATURES
ROLES
ARRAY
RNAS
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
13.1
Papers:
3.6W
Citations:
29.0W
Organization
Cited Papers
ShareSaveThe muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2.png)
NATURE MEDICINE
NATURE MEDICINEIF50
PREAI
ShareSaveShareSaveShareSaveThe MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurementsNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAIShareSaveShareSaveShareSave
View more