Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS

doi:10.1016/j.chroma.2025.466440

Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS

2025-10-04

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DOI：10.1016/j.chroma.2025.466440

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Abstract

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• Analytical hydrophobic interaction chromatography combined with LC-MS to evaluate hydrophobicity of CHO cell proteins • GRAVY number provides very poor prediction of HCP hydrophobicity • Calculations using 3D protein structure calculated by AlphaFold in better agreement with measured hydrophobicity • Results demonstrate effectiveness of analytical HIC coupled with LC-MS to explore hydrophobicity of complex mixtures

Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS

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