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Imaging across multiple spatial scales with the multi-camera array microscope
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DOI：10.1364/OPTICA.478010.png)
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Abstract
En 中文
This paper experimentally examines different configurations of a multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon a densely packed array of micro-cameras to jointly image across a large field -of-view (FOV) at high resolution. Each micro-camera within the array images a unique area of a sample of interest, and then all acquired data with 54 micro-cameras are digitally combined into composite frames, whose total pixel counts significantly exceed the pixel counts of standard microscope systems. We present results from three unique MCAM configurations for different use cases. First, we demonstrate a configuration that simultaneously images and estimates the 3D object depth across a 100 x 135 mm(2) FOV at approximately 20 mu m resolution, which results in 0.15 gigapixels (GP) per snapshot. Second, we demonstrate an MCAM configuration that records video across a continuous 83 x 123 mm(2) FOV with twofold increased resolution (0.48 GP per frame). Finally, we report a third high-resolution configuration (2 mu m resolution) that can rapidly produce 9.8 GP composites of large histopathology specimens. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
Keywords:
FOURIER PTYCHOGRAPHIC MICROSCOPY
CELLULAR RESOLUTION
HIGH-THROUGHPUT
WIDE-FIELD
INSPECTION
LIMIT