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Localization microscopy at doubled precision with patterned illumination

delete2019-12-09
delete138
delete
OA
AI
J
Jelmer Cnossen
T
Taylor Hinsdale
R
Rasmus Ø. Thorsen
M
Marijn Siemons
F
Florian Schueder
R
Ralf Jungmann
C
Carlas Smith *
B
Bernd Rieger
S
Sjoerd Stallinga *
DOI：10.1038/s41592-019-0657-7delete
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Abstract

Abstract

En 中文
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging. SIMFLUX combines elements of MINFLUX with structured illumination to double localization precision and improve resolution in localization microscopy. The approach was demonstrated on DNA origami and on cellular microtubules.
Keywords:
SINGLE-MOLECULE LOCALIZATION
SUPERRESOLUTION MICROSCOPY
RESOLUTION
NANOSCOPY
TRACKING
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Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Nature Methods cover
Nature Methods
IF:
32.1
Papers:
7.2K
Citations:
12.7W

Organization

U
Utrecht University
Scholars:
6.0W
Papers: 5.1W
Citations: 5.8W
D
Delft University of Technology
Scholars:
2.6W
Papers: 2.5W
Citations: 3.8W
U
University of Munich
Scholars:
5.7W
Papers: 4.2W
Citations: 68
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