.png){kind=icon}
Return
Machine learning–directed massively parallel programmable nucleic acid amplification
.png){kind=icon}
OA
AI
DOI：10.1126/sciadv.aec9175.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Dynamic regulation of amplification efficiency is pivotal yet challenging in molecular diagnostics and DNA data storage. Here, we develop a thermodynamics-based approach to achieve continuous and precise modulation of nucleic acid amplification efficiency. By decoupling sequence specificity from hybridization energy regulation via a primer-tag compensation strategy, we demonstrate programmed amplification with high resolution (33 versus 81%). Leveraging 2483 experimental data, we constructed a machine learning model that improved prediction accuracy from R2 = 0.62 to = 0.86. In DNA data storage, this amplification strategy increases the density for information preview by nearly one order of magnitude and robust file steganography via differential amplification. In clinical validation, our method outperformed uniform amplification in cervical cancer RNA variant analysis, detecting rare RNA fusions and improving detection sensitivity by 100-fold under 104 simulated sequencing depth. This programmable technique is anticipated to extend to single-cell sequencing and spatial transcriptomics, offering a powerful tool for molecular diagnostics and synthetic biology.
Keywords:
Nucleic acid amplification
Machine learning
DNA data storage
Molecular diagnostics
Programmable amplification
Journal
IF:
12.5
Papers:
2.1W
Citations:
18.1W
Organization
View more organizations
Cited Papers
Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS).png)
NATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAIShareSaveA deep learning model for predicting next-generation sequencing depth from DNA sequenceNATURE COMMUNICATIONS
OAAI
NATURE COMMUNICATIONSIF15.7
OAAIShareSaveMolecular-level similarity search brings computing to DNA data storageNATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAIShareSaveSimulation-guided DNA probe design for consistently ultraspecific hybridizationNATURE CHEMISTRYOAAI
NATURE CHEMISTRYIF20.2
OAAIShareSaveShareSaveShareSaveShareSave View more