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Profiling protein function with small molecule microarrays
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DOI：10.1073/pnas.172286899.png)
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Abstract
En 中文
The regulation of protein function through posttranslational modification, local environment, and protein-protein interaction is critical to cellular function. The ability to analyze on a genome-wide scale protein functional activity rather than changes in protein abundance or structure would provide important new insights into complex biological processes. Herein, we report the application of a spatially addressable small molecule microarray to an activity-based profile of proteases in crude cell lysates. The potential of this small molecule-based profiling technology is demonstrated by the detection of caspase activation upon induction of apoptosis, characterization of the activated caspase, and inhibition of the caspase-executed apoptotic phenotype using the small molecule inhibitor identified in the microarray-based profile.
Keywords:
GENE-EXPRESSION
GRANZYME-B
MASS-SPECTROMETRY
PROSTATE-CANCER
SPECIFICITY
INHIBITORS
APOPTOSIS
DNA
FRAGMENTATION
LIBRARIES
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9.1
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10.8W
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73.5W
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