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Rapid nonlinear image scanning microscopy
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DOI：10.1038/NMETH.4467.png)
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Abstract
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Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.
Keywords:
STRUCTURED-ILLUMINATION MICROSCOPY
INTACT MOUSE-BRAIN
2ND-HARMONIC GENERATION
CONFOCAL MICROSCOPY
FLUORESCENCE MICROSCOPY
LIVE CELLS
RESOLUTION
COLLAGEN
REASSIGNMENT
IMPROVES
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