.png){kind=icon}
Return
Real-time high dynamic range laser scanning microscopy
.png){kind=icon}
OA
AI
DOI：10.1038/ncomms11077.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem of selective data display, we developed a new method that extends the imaging dynamic range in optical microscopy and improves the signal-to-noise ratio. Here we demonstrate how real-time and sequential high dynamic range microscopy facilitates automated three-dimensional neural segmentation. We address reconstruction and segmentation performance on samples with different size, anatomy and complexity. Finally, in vivo real-time high dynamic range imaging is also demonstrated, making the technique particularly relevant for longitudinal imaging in the presence of physiological motion and/or for quantification of in vivo fast tracer kinetics during functional imaging.
Keywords:
SINGLE-CELL
HIGH-THROUGHPUT
SENSITIVITY
SCALE
DYES
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
15.7
Papers:
9.4W
Citations:
91.2W
Organization
Cited Papers
High-throughput, detailed, cell-specific neuroanatomy of dendritic spines using microinjection and confocal microscopy.png)
NATURE PROTOCOLSOAAI
NATURE PROTOCOLSIF16
OAAIShareSaveThe regulation of mitochondrial morphology: Intricate mechanisms and dynamic machineryCELLULAR SIGNALLING
CELLULAR SIGNALLINGIF3.7
PREAI
ShareSaveShareSaveShareSaveWhole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotypingNATURE PROTOCOLSOAAI
NATURE PROTOCOLSIF16
OAAIShareSaveShareSave
View more