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Removal of Soluble Nitrogen From Surimi Wash Water by Protein Recovery Using Lactic Acid for Safe Discharge
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DOI：10.1111/1750-3841.71257.png)
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Abstract
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In the present study, the effects of pH (2, 3, 4, 5, and 6) and incubation time (0, 10, 20, and 30 min) on the removal of soluble nitrogen from surimi wash water by recovery of protein were investigated. The highest protein removal rate was recorded at pH 4 at 0-10 min (90.43%), was optimized, and further used for the comparison with industrial untreated (raw) surimi water and centrifuged surimi wash water. Acid treated sample showed lower values of protein (0.1%) and fat (0.1%) followed by centrifuged surimi wash water. The significantly (p < 0.05) higher biochemical oxygen demand (BOD) (320 mg/L) and chemical oxygen demand (COD) (800 mg/L) were observed in raw wash water (RWW) and acid treated wash water compared to centrifuged wash water (CWW). The lowest value of ammoniacal nitrogen (24.8 mg/L), total phosphorus (92.8 mg/L), and total suspended solids (4 mg/L) were found in treated surimi wash water. Samples treated with acids (pH 4) (using lactic acid as a green organic acid) notably showed significantly higher values of amino acids compared to RWW. SDS analysis indicated that the treated surimi wash water with acidic condition significantly reduced protein band intensity on SDS–PAGE compared to the other due to higher protein removal and other organic compounds. The lowest microbial loads such as Aerobic plate count (3.6 × 105 CFU/mL), yeast and mould counts (2.1 × 104 CFU/mL) and presence of Lactobacillus (84 CFU/mL) was associated with acid treated surimi wash water.
Keywords:
amino acid profiling
biochemical oxygen demand (BOD)
chemical oxygen demand (COD)
lactic acid precipitation
lactic acid precipitation
protein recovery
SDS-PAGE
surimi wash water
total suspended solids
wastewater treatment
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