Selective-plane-activation structured illumination microscopy

doi:10.1038/s41592-024-02236-3

Selective-plane-activation structured illumination microscopy

2024-04-05

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DOI：10.1038/s41592-024-02236-3

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The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids. The combination of light sheet illumination and reversibly switchable fluorophores enables improved structured illumination microscopy for fast, low-background super-resolution imaging in cells and spheroids.

Keywords:

RESOLUTION LIMIT

ADAPTIVE OPTICS

ENHANCEMENT

DEEP

Selective-plane-activation structured illumination microscopy

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