Simultaneous multicolor fluorescence imaging using PSF splitting

doi:10.1038/s41592-024-02383-7

Simultaneous multicolor fluorescence imaging using PSF splitting

2024-09-06

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Thomas Schlichthaerle

DOI：10.1038/s41592-024-02383-7

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We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the 'Circulator', an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view. Point spread function (PSF) splitting with the 'Circulator', which encodes the fluorophore emission band into the PSF, improves the information content of fluorescence microscopy and enables improved super-resolution imaging and single-particle tracking.

Keywords:

LOCALIZATION MICROSCOPY

SUPERRESOLUTION

TRACKING

SYK

Simultaneous multicolor fluorescence imaging using PSF splitting

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