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Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array
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DOI：10.1002/lpor.202100026.png)
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Abstract
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Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high-numerical-aperture (NA) objectives or requires specially designed long-working-distance objectives. Here, a LSFM based on a micro-mirror array (MMA) to enable light sheet imaging with a single objective lens is reported. The planar fluorescent emission excited by the light sheet illumination is collected by the same objective, relayed onto an MMA and detected by a side-view camera. The proposed scheme makes LSFM compatible to single objective imaging system and shows promising candidacy for high spatiotemporal imaging.
Keywords:
light-sheet fluorescence microscopy
micro-mirror arrays
single-lens
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