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Single-shot 20-fold expansion microscopy
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DOI：10.1038/s41592-024-02454-9.png)
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Abstract
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Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (similar to 4-10x linearly) or achieve larger expansion factors through iterating the expansion process a second time (similar to 15-20x linearly). Here, we present an ExM protocol that achieves similar to 20x expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.
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