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spaCl: deciphering spatial cellular communications through adaptive graph model
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DOI：10.1093/bib/bbac563.png)
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Abstract
En 中文
Cell-cell communications are vital for biological signalling and play important roles in complex diseases. Recent advances in single cell spatial transcriptomics (SCST) technologies allow examining the spatial cell communication landscapes and hold the promise for disentangling the complex ligand-receptor (L-R) interactions across cells. However, due to frequent dropout events and noisy signals in SCST data, it is challenging and lack of effective and tailored methods to accurately infer cellular communications. Herein, to decipher the cell -to -cell communications from SCST profiles, we propose a novel adaptive graph model with attention mechanisms named spaCl. spaCl incorporates both spatial locations and gene expression profiles of cells to identify the active L-R signalling axis across neighbouring cells. Through benchmarking with currently available methods, spaCl shows superior performance on both simulation data and real SCST datasets. Furthermore, spaCl is able to identify the upstream transcriptional factors mediating the active L-R interactions. For biological insights, we have applied spaCl to the seqFISH data of mouse cortex and the NanoString CosMx Spatial Molecular Imager (SMI) data of non -small cell lung cancer samples. spaCl reveals the hidden L-R interactions from the sparse seqFISH data, meanwhile identifies the inconspicuous L-R interactions including THBS1-ITGB1 between fibroblast and tumours in NanoString CosMx SMI data. spaCl further reveals that SMAD3 plays an important role in regulating the crosstalk between fibroblasts and tumours, which contributes to the prognosis of lung cancer patients. Collectively, spaCl addresses the challenges in interrogating SCST data for gaining insights into the underlying cellular communications, thus facilitates the discoveries of disease mechanisms, effective biomarkers and therapeutic targets.
Keywords:
single -cell spatial transcriptomics
spatial cell graph
adaptive graph model
triplet loss
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