.png){kind=icon}
.png){kind=icon}
Return
Supermultiplexed optical imaging and barcoding with engineered polyynes
.png){kind=icon}
OA
AI
DOI：10.1038/NMETH.4578.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Optical multiplexing has a large impact in photonics, the life sciences and biomedicine. However, current technology is limited by a 'multiplexing ceiling' from existing optical materials. Here we engineered a class of polyyne-based materials for optical supermultiplexing. We achieved 20 distinct Raman frequencies, as 'Carbon rainbow', through rational engineering of conjugation length, bond-selective isotope doping and end-capping substitution of polyynes. With further probe functionalization, we demonstrated ten-color organelle imaging in individual living cells with high specificity, sensitivity and photostability. Moreover, we realized optical data storage and identification by combinatorial barcoding, yielding to our knowledge the largest number of distinct spectral barcodes to date. Therefore, these polyynes hold great promise in live-cell imaging and sorting as well as in high-throughput diagnostics and screening.
Keywords:
STIMULATED RAMAN-SCATTERING
THROUGHPUT MULTIPLEXED DETECTION
FLOW-CYTOMETRY
LIVE CELLS
DNA
MICROSPHERES
SPECTROSCOPY
BIOMOLECULES
MICROSCOPY
SYSTEMS
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.