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Tomographic phase microscopy

delete2007-08-12
delete918
PRE
AI
W
Wonshik Choi
C
Christopher Fang‐Yen
K
Kamran Badizadegan
S
Seungeun Oh
N
Niyom Lue
R
Ramachandra R. Dasari
M
Michael S. Feld *
DOI：10.1038/NMETH1078delete
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Abstract

Abstract

En 中文
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Keywords:
DIGITAL HOLOGRAPHIC MICROSCOPY
REFRACTIVE-INDEX
RESOLUTION
CELLS
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AI Summary

Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Nature Methods cover
Nature Methods
IF:
32.1
Papers:
7.2K
Citations:
12.7W

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