.png){kind=icon}
.png){kind=icon}
Return
Transcriptome Analysis of Mycobacteria-Specific CD4+ T Cells Identified by Activation-Induced Expression of CD154
.png){kind=icon}
OA
AI
DOI：10.4049/jimmunol.1700654.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Analysis of Ag-specific CD4(+) T cells in mycobacterial infections at the transcriptome level is informative but technically challenging. Although several methods exist for identifying Ag-specific T cells, including intracellular cytokine staining, cell surface cytokine-capture assays, and staining with peptide:MHC class II multimers, all of these have significant technical constraints that limit their usefulness. Measurement of activation-induced expression of CD154 has been reported to detect live Ag-specific CD4(+) T cells, but this approach remains underexplored and, to our knowledge, has not previously been applied in mycobacteria-infected animals. In this article, we show that CD154 expression identifies adoptively transferred or endogenous Ag-specific CD4(+) T cells induced by Mycobacterium bovis bacillus Calmette-Guerin vaccination. We confirmed that Ag-specific cytokine production was positively correlated with CD154 expression by CD4(+) T cells from bacillus Calmette-Guerin vaccinated mice and show that high quality microarrays can be performed from RNA isolated from CD154(+) cells purified by cell sorting. Analysis of microarray data demonstrated that the transcriptome of CD4(+) CD154(+) cells was distinct from that of CD154 cells and showed major enrichment of transcripts encoding multiple cytokines and pathways of cellular activation. One notable finding was the identification of a previously unrecognized subset of mycobacteria-specific CD4(+) T cells that is characterized by the production of IL-3. Our results support the use of CD154 expression as a practical and reliable method to isolate live Ag-specific CD4(+) T cells for transcriptomic analysis and potentially for a range of other studies in infected or previously immunized hosts.
Keywords:
FOLLICULAR HELPER-CELLS
MAST-CELL
TERMINAL DIFFERENTIATION
ACUTE-INFLAMMATION
DENDRITIC CELL
INFECTION
IMMUNITY
MICE
IL-3
BASOPHILS
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.