.png){kind=icon}
.png){kind=icon}
Return
Transcriptomic and functional profiling of human intestinal organoids identifies enhanced calcium signalling and thrombospondin-2 activity in cystic fibrosis
.png){kind=icon}
OA
AI
E
W
N
S
R
M
DOI：10.1016/j.jcf.2026.06.007.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
• CRISPR-edited human organoids reliably model intestinal epithelial cells in cystic fibrosis. • CFTR knockout organoids capture disease-related phenotypes at a molecular and functional level. • Loss of CFTR results in elevated Ca2+ flux, which may be associated with a sensitised THBS2 response. • Verapamil provides in vitro protection against elevated Ca2+ and THBS2 in CFTR knockout organoids.
Keywords:
Human intestinal organoids
Cystic fibrosis
CRISPR
Calcium dysregulation
CFTR
WT
Wild-type
KO
Knockout
HIO
Human intestinal organoid
IEO
Intestinal epithelial organoid
CF
Cystic fibrosis
FIS
Forskolin-induced swelling
CRC
Colorectal cancer
SC
Sigmoid colon
DEG
Differentially expressed gene
hDEG
Highly differentially expressed gene
GSEA
Gene-set enrichment analysis
TA
Transit-amplifying
VER
Verapamil
ROS
Reactive oxygen species
TEER
Transepithelial electrical resistance
EMT
Epithelial-to-mesenchymal transition
ROI
Region of interest
Journal
IF:
6
Papers:
2.9K
Citations:
7.3K
Organization
Cited Papers