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Protein Expression and Purification

IF1.2
论文数69
被引数4879
期刊论文 69
发表时间
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Comparative structural analysis of Nipah virus V proteins highlights V-MDA5 complex stability in pig and bat isolates尼帕病毒V蛋白的结构比较分析强调了猪和蝙蝠分离株中V-MDA5复合物的稳定性
deleteProtein Expression and Purification
IF1.2
delete2026-09-01
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PREAI
deleteChong, Chee Ning; Ong, Hui Ming; Liew, Yvonne Jing Mei; Lee, Yean Kee; Jonet, Mohd Anuar; Chang, Li-Yen
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SUMO-assisted expression of a soluble and functional Venus flytrap domain of the human sweet taste receptor TAS1R2 in Escherichia coliSUMO辅助表达可溶且功能性的人类甜味受体TAS1R2的捕蝇夹结构域于大肠杆菌中
deleteProtein Expression and Purification
IF1.2
delete2026-09-01
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deleteBelloir, Christine; Karolkowski, Adeline; Moitrier, Lucie; Briand, Loic
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Molecular chaperones and solubility tags: A 44-fold increase in recombinant fibEase yield for therapeutic applications分子伴侣和溶解性标签：用于治疗应用的重组fibEase产量提高44倍
deleteProtein Expression and Purification
IF1.2
delete2026-07-01
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PREAI
deleteChe, Zhiqun; Wei, Suyuan; Sun, Renjie; Guo, Chun
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Extraction, purification and characterization of lipoxygenase from foxtail millet (Setaria italica)从谷子(Setaria italica)中提取、纯化及鉴定脂氧合酶
deleteProtein Expression and Purification
IF1.2
delete2026-05-01
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PREAI
deleteLi, Chen; Tan, Zonghua; Li, Jiao; Liu, Sining; Cui, Xiaodong; Zhang, Lizhen
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High yield production of C-terminally processed KRAS4a, HRAS, and NRAS for biophysical studyC-末端加工的KRAS4a、HRAS和NRAS的高产率制备用于生物物理研究
deleteProtein Expression and Purification
IF1.2
delete2026-05-01
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deletePerkins, Shelley; Krahnke, Sophie; Larsen, Erik K.; Drew, Matthew; Frank, Peter H.; Hong, Min; Rabara, Dana; Wall, Vanessa E.; Grose, Carissa; Gillette, William; Esposito, Dominic; Stephen, Andrew G.; Messing, Simon A.
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A novel flavin-containing monooxygenase from Pseudomonas guineae enables efficient biosynthesis of indigo from indole via both enzymatic and cell factory approaches来自Pseudomonas guineae的一种新型黄素依赖性单加氧酶通过酶法和细胞工厂方法实现了吲哚向靛蓝的高效生物合成
deleteProtein Expression and Purification
IF1.2
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deleteHao, Zhebin; Zhang, Yulei; Zhang, Wenli; Zhu, Yingying; Mu, Wanmeng
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Catalytically active hCELA3B is a natively-folded monomer and is N-glycosylated (vol 238, 106840, 2026)催化活性的hCELA3B是天然折叠的单体且被N-糖基化（卷238，106840，2026）
deleteProtein Expression and Purification
IF1.2
delete2026-05-01
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deleteKumar, Prince; Babele, Prabhakar; Madhukalya, Rishav; Goswami, Abhishek; Khan, Khadijah Ameen; Gupta, Rohit; Dadwal, Anica; Kumar, Rajesh; Kumar, Dilip; Garg, Pramod Kumar; Das, Supratik
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Protein expression control by targeted elimination of ovomucoid in chicken eggs通过靶向消除鸡卵中的卵粘蛋白来控制蛋白质表达
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IF1.2
delete2026-05-01
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deleteEzaki, Ryo; Horiuchi, Hiroyuki
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Sterile chicken hosts for germline transmission: A review of recipient preparation strategies in avian biotechnology无菌鸡作为生殖系传播的宿主：禽类生物技术中受体准备策略的综述
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IF1.2
delete2026-05-01
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deleteChen, Yi-Chen; Takemoto, Tatsuya
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Partial purification and characterization acidophilic lipase from Bacillus cereus ALP E1枯草芽孢杆菌ALP E1酸性脂肪酶的部分纯化与表征
deleteProtein Expression and Purification
IF1.2
delete2026-03-01
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deleteSari, Nindy Novita; Nurhasanah; Haryati, Titin
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Strategies for manufacturing soluble human alphainterferons in Escherichia coli: expression, purification, and testing在埃希氏 coli 中制备可溶性人α干扰素的策略：表达、纯化和测试
deleteProtein Expression and Purification
IF1.2
delete2026-02-01
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deleteBretas, Rodrigo Martins; Leclercq, Sophie Yvette; Cunha Jr, Armando da Silva; Lopes, Luciana Maria Silva
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Hyper-expression of functional human basic fibroblast growth factor 2 in Escherichia coli功能性人碱性成纤维细胞生长因子2在埃希氏大肠杆菌中的高表达
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IF1.2
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deleteManavalan, Tamilvendan; Bi, Jiawu; Naulchan, Khongsay; Van, Nguyen Thi Bich; Chua, Xin Yi; Yow, Ryan; Lee, Sook Yee; Chew, Lamony Jian Ming; Ottenheim, Christoph; Sugii, Shigeki; Lakshmanan, Manikandan; Zhou, Weibiao; Arumugam, Prakash; Wong, Fong Tian; Weingarten, Melanie; Ang, Ee Lui
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Removal of polysorbates degradation-related impurities by depth filter screening: A case study通过深层过滤器筛选去除聚山梨酯降解相关杂质：一个案例研究
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IF1.2
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deleteXuan, Chunxiao; Xu, Yangguang; Cui, Xinang; Ma, Tengfei; Lv, Fengjuan
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Expression, purification, and reconstitution of small-conductance mechanosensitive channel into lipid bilayer: ready for solid-state NMR study小电导机械敏感性通道的表达、纯化及脂质体重构：已准备好进行固态核磁共振研究
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IF1.2
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deleteMeng, Shenghua; Gao, Zixiang; Lin, Yuan; Zhang, Yan; Xie, Huayong; Yang, Jun; Tong, Qiong
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Recombinant expression and purification of TANB77-derived pilin-like proteins 935 and 938TANB77衍生菌毛样蛋白935和938的重组表达与纯化
deleteProtein Expression and Purification
IF1.2
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deleteTran, Tham Truong Phuong; Nguyen, Linh Thanh; Han, Byeong-Gu; Lee, Min-Jung; Kim, Byoung Choul
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A self-cleavable ULP1-SUMO fusion strategy for enhanced soluble protein expression and one-step purification in Escherichia coli一种用于增强可溶性蛋白质表达和一步纯化的自切割ULP1-SUMO融合策略在埃希氏菌属大肠杆菌中的应用
deleteProtein Expression and Purification
IF1.2
delete2026-02-01
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deleteJin, Changbin; Zhang, Xiaohu; Yang, Zhibo; Yuan, Wei; Shi, Hao; Zhu, Jingru; Zhang, Yueping
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Robust aggregate separation by Sartobind Rapid A Protein A membraneSartobind Rapid A 蛋白A膜实现的稳健团聚物分离
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IF1.2
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deleteYuan, Gaoya; Qu, Meng; Li, Yifeng
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Bioinformatics analysis and preparation of polyclonal antibodies against the extracellular region of the Langya Virus attachment protein生物信息学分析及抗Langya病毒附着蛋白胞外区的多克隆抗体制备
deleteProtein Expression and Purification
IF1.2
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deleteQiu, Shuxing; Yin, Xing; He, Jinjiao; Zhang, Jiayou; Guo, Jinjing; Qi, Yonghua; Liu, Feifan
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Scalable production and characterization of recombinant pneumococcal histidine triad protein D in Escherichia coli for vaccine development重组肺炎链球菌组氨酸三联蛋白D在埃希氏大肠杆菌中的规模化生产和表征，用于疫苗开发
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IF1.2
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deleteJadhav, Prashant; Malviya, Hitesh; Gokhale, Jyoti
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Purification and characterization of the peroxiredoxin 5-thioredoxin 2 complex: unraveling the redox regeneration of peroxiredoxin 5 by thioredoxin 2 within the peroxiredoxin catalytic cycle过氧化物还原酶5-硫氧还蛋白2复合物的纯化与表征：揭示过氧化物还原酶5在催化循环中通过硫氧还蛋白2的氧化还原再生
deleteProtein Expression and Purification
IF1.2
delete2026-01-01
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deleteYang, Xiangwei; Yang, Yifan; Gong, Sizhe; Wang, Li; Liu, Qian; Sun, Ruiqi; Lian, Fu-Ming
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