杨晓兰 (Xiaolan Yang)

Chongqing Medical University教授、硕士生导师、博士生导师邮箱:xiaolanyang666@cqmu.edu.cn

H指数:27

被引数:2,401

个人简介

"杨晓兰，女，药理学博士,教授，博士生导师\n■联系信息\n重庆医科大学检验医学院\n临床检验诊断学教育部重点实验室\n办公电话, +86-23-68485240;\nE-mail, xiaolanyang666@cqmu.edu.cn\nhttp:// orcid.org/0000-0001-9320-1036\nQQ: 229169980, TEL: 15909319667\n■学习与工作经历\n1987年9月～1991年7月西南师范大学化学系理学学士\n1991年9月～1994年7月北京师范大学有机化学专业理学硕士\n2003年9月～2006年7月重庆医科大学药理学专业博士\n1994年7月～2003年7月重庆医科大学基础医学院化学教研室任教\n2003年7月～2010年12月重庆医科大学药学院药化教研室任教\n2011年1月～至今，重庆医科大学检验医学院、临床检验诊断学教育部重点实验室教授、硕士生导师、博士生导师\n2021年12月～2022年3月，香港科技大学化学系访问学习\n■研究方向\n1.疾病标志物高灵敏快速检测新方法和生物传感构建、荧光量子点新材料制备及应用；\n2.磁分离选择性指数富集快速筛选混合物中高亲和力配体。\n■科研项目\n作为负责人主持的科研项目主持国家自然科学面上项目2项，863计划子课题1项，省部课题3项，厅局级2项\n1.2023/1-2026/12 国家自然科学基金面上项目“反复非平衡结合联合迭代筛选发掘混合物中痕量高亲和力配体的方法及其应用”编号82273900，在研。\n2.2023/7-2026/06 重庆市科技局自然科学基金(面上项目) “生理缓冲液中稳定分散的表面功能化铅卤铯钙钛矿量子点合成及呼吸道病原体多重检测”编号CSTB2023NSCQ- MSX0994，在研。\n3.2021/10-2024/09重庆市渝中区科学技术局基础研究与前沿探索基于Cas13a双级联循环剪切非扩增快速检测RNA方法及在多种呼吸道RNA病毒快检中的应用，编号20210132。\n4.2019/07-2022/06重庆市科委面上项目“指数富集快速发现天然产物混合物中抗结核活性先导配体”，编号CSTC2019jcyj-msxmX0166。\n5.2016/01-2019/12，国家自然科学基金面上项目“混合物中低丰度高亲和力配体的指数富集技术及应用”，编号31570862。\n6.2012/09-2015/09，重庆市科委面上项目“融合表达标签GST亲和标记试剂设计筛选与应用”，编号CSTC2012JJA0057。\n7.2011/09-2012/12，国家高技术研究发展计划（863计划,编号2011AA02A108）子课题6 “新型试剂材料制备”。\n8.2010/06-2012/12，重庆市教委项目“聚乙二醇包被磁性微球制备及其在配体混合物组合库筛选中的应用”，编号KJ100313。\n■近5年代表性学术论著\n1.Yang,X.;Xie,Y.,Zhong,Y.;Li,Y.,Bin, W.,Liao,L.,Qi, G.;Zuo,L.,Li,S.*;Yang,X*.Highly responsive push-orchestrated-pull switching-enabled RNA-regulated Cas12a cis-cleavage: A universal one-pot multiplex-targeted detection platform with application to Mycobacterium tuberculosis complex,Chemical Engineering Journal,2025,523:168126.\n2.Al-Dulaiei, M.,Ma, T.,Long, J.,Yu, Y.,Li, Y.,Yang,X.*Hairpin reporter facilitated single cas13a test for amplification-free highly sensitive detection of mirna-424, Microchemical Journal 2025,213:113768.\n3.Liao,L.(co-first);Xie,Y.(co-first);Qi, G.,Wu, Z.,Wu, Y.;Yang,X.,He, Q.,Li, Y.,Zhong,Y.,Yang,X.*One-pot rapid detection of microRNA based on a double-hairpin templated exponential isothermal amplification with positive feedback,Microchemical Journal,2025,217:114991.\n4.Long, J.(co-first), Liu, Q.(co-first), AL-Dulaiei, M.(co-first), Xie, Y., Chen, M., Wu, Z.,Wu,Yu.,Yang, X.*,One-step detection of miRNA-21 based on self-priming hairpin-mediated isothermal amplification coupled with DNAzyme.Microchemical Journal,2025,113548.\n5.Wu, Z., Wu, Y., Niu, Y., Hu, Q., Jiang, Q., Liao, L.,Qi, G., Lan, H.,Yang, X.*,Discovery of novel inhibitors for malate synthase of Mycobacterium Tuberculosis from natural products.Bioorganic & Medicinal Chemistry Letters,2025,123:130217.\n6.Niu, Y., Wu, Z., Hu, Q., Wu, Y., Jiang, Q,Yang, X.*, Discovery of acetohydroxyacid synthase inhibitors as anti-tuberculosis lead compounds from natural products. Bioorganic & Medicinal Chemistry, 2025, 118:118041.\n7.Chen, M.(co-first), Jiang, X(co-first)., Hu, Q., Long, J., He, J., Wu, Y., Wu, Z., Niu, Y., Jing, C.,Yang, X.*, Toehold-Containing Three-Way Junction-Initiated Multiple Exponential Amplification and CRISPR/Cas14a Assistant Magnetic Separation Enhanced Visual Detection ofMycobacterium Tuberculosis.ACS Sensors, 2024, 9(1):62-72.\n8.Wu, Q., Yu, Y., Chen, M., Long, J.,Yang, X.*, A label-free fluorescence sensing strategy based on GlaI-assisted EXPAR for rapid and accurate quantification of human methyltranferase activity. Talanta, 2024, 269:125456.\n9.Xie, Y., Hou, Y., Yu, Y., Zhang, J., Long, J., Chen, M., Lang, X.,Yang, X.*, Chen, H*., Cascade branch migration-triggered strand displacement amplification for specific and sensitive detection of microRNA. Analytical Methods, 2024, 16(25):4116-4123.\n10.Long, J., Chen, M., Yu, Y., Wu, Q.,Yang, X.*, Triple-recognition strategy for one-pot detection of single nucleotide variants by aligner-mediated cleavage-triggered exponential amplification. Analytica Chimica Acta, 2023, 1276:341617.\n11.Wu, Q., Xiang, X., Yuan, Y., Yu, Y., Chen, M., Long, J., Xiang, T.*,Yang, X.*, GlaI cleavage assistant isothermal exponential amplification coupling with CRISPR/Cas12a for ultrasensitive detection of CLDN11 methylation: A potential marker for lung adenocarcinoma. Sensors and Actuators B: Chemical, 2023, 385:133675.\n12.Jiang, X., Zeng, H., Duan, C., Hu, Q., Wu, Q., Yu, Y.,Yang, X.*, One-pot synthesis of stable and functional hydrophilic CsPbBr3perovskite quantum dots for“turn-on”fluorescence detection ofMycobacterium tuberculosis.Dalton Transactions, 2022, 51(9):3581-3589.\n13.Zeng, H., Zhang, P., Jiang, X., Duan, C., Yu, Y., Wu, Q.,Yang, X.*, Rapid RNA detection through intra-enzyme chain replacement-promoted Cas13a cascade cyclic reaction without amplification. Analytica Chimica Acta, 2022, 1217:340009.\n14.Yu, Y., Zeng, H., Wu, Q., Jiang, X., Duan, C., Long, J., Chen, M.,Yang, X.*, A sensing strategy combining T7 promoter-contained DNA probe with CRISPR/Cas13a for detection of bacteria and human methyltransferase. Analytica Chimica Acta, 2022, 1227:340266.\n15.Duan, C., Jiang, Q., Jiang, X., Zeng, H., Wu, Q., Yu, Y.,Yang, X.*,Discovery of a Novel Inhibitor Structure ofMycobacterium tuberculosisIsocitrate Lyase. Molecules, 2022, 27(8):2447.\n16.Xu, B., Tong, T., Wang, Xin., Liu, Fang., Zhang, X., Hu, X., Li, X.,Yang, X.*, Liao, F.*,Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers. Journal of Enzyme Inhibition and Medicinal Chemistry, 2022, 37(1):728-742.\n17.Li X., Jiang Q*.,Yang X.*, Discovery of inhibitors for Mycobacterium tuberculosis peptide deformylase based on virtual screening in silico. Molecular Informatics. 2021, 40, 2100002. DOI: 10.1002/minf.202100002\n18.Chen, T., Yang, L.,Yang, X*., Liao, F.*, Ion-exchangemedium coated with abundant small zwitterions for the purification of soluble proteins. Prep Biochem Biotech, 2021, 51(4):405-413.\n■专利授权\n1.杨晓兰，段昌园。一种结核分枝杆菌异柠檬酸裂解酶先导抑制剂，国家发明专利，ZL202210217785.2，授权日期2024-7-23。\n2.杨晓兰，姜雪，胡小蕾。一种功能化亲水性铅卤钙钛矿量子点制备及生物传感应用，ZL202110536658.4，授权日期2023-03-20。\n3.杨晓兰，夏超，廖飞，胡小蕾，张祥。标签GST的一种不可逆亲和标记试剂及应用，ZL201710000094.6，授权日期2020-02-04。\n4.杨晓兰，何姝，廖飞"