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Adaptable, illumination patterning light sheet microscopy
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DOI：10.1038/s41598-018-28036-2.png)
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摘要
En 中文
Minimally-invasive optical imaging requires that light is delivered efficiently to limit the detrimental impact of photodamage on delicate biological systems. Light sheet microscopy represents the exemplar in tissue specific optical imaging of small and mesoscopic samples alike. However, further gains towards gentler imaging require a more selective imaging strategy to limit exposure to multiple yet discrete tissues without overexposing the sample, particularly where the information content is sparse or particularly optically sensitive tissues are present. The development of sample-adaptive imaging techniques is crucial in pursuit of the next generation of smart, autonomous microscopes. Herein, we report a microscope capable of performing 4D (x, y, z, t) light patterning to selectively illuminate multiple, rapidly reconfigurable regions of interest while maintaining the rapid imaging speed and high contrast associated with light sheet microscopy. We illustrate this utility in living zebrafish larvae and phantom samples.
Keyword:
FLUORESCENCE MICROSCOPY
HIGH-SPEED
LONG-TERM
RECONSTRUCTION
PHOTODAMAGE
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对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.9
论文数:
27.9W
被引数:
83.5W
机构
引用论文
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OAAI
OAAI分享收藏Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.png)
NATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏Major signal increase in fluorescence microscopy through dark-state relaxationNATURE METHODSOAAI
NATURE METHODSIF32.1
OAAI分享收藏Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organismsNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscopeDEVELOPMENTOAAI
DEVELOPMENTIF3.6
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