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Adaptive optics improves multiphoton super-resolution imaging
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DOI：10.1038/NMETH.4337.png)
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摘要
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We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 +/- 10 nm laterally, 729 +/- 39 nm axially) at depths similar to 250 mu m from the coverslip surface.
Keyword:
STRUCTURED ILLUMINATION MICROSCOPY
RESOLUTION
ABERRATIONS
LIVE
DIVERSITY
CELLS
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32.1
论文数:
7.2K
被引数:
12.7W
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