Can protein expression be ‘solved’?

Catherine Baranowski

doi:10.1016/j.tibtech.2025.04.021

2025-06-02

Christopher J. Petzold

Evangelos-Marios Nikolados

Erika A. DeBenedictis *

DOI：10.1016/j.tibtech.2025.04.021

原文链接

查看原文

摘要

En 中文

异源蛋白质表达是现代生物学中经常使用的基本技术。它可以对蛋白质功能进行科学探索，并开发救生药物和具有经济影响力的工业产品。蛋白质表达实验主要仍然是一种经验指导的试错情况，尽管它是几乎所有生物学家都使用的方法。生成一个跨越生物体并使用标准实验方法的公开可用的大规模蛋白质表达数据集将为机器学习社区提供构建多物种表达预测模型的基础。蛋白质表达的预测模型将具有深远的商业影响，并且可以用更高概率的定向方法代替无数小时的实验。

Keyword:

protein expression

machine learning

predictive models

open datasets

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Trends in Biotechnology

IF:

14.9

论文数:

3.8K

被引数:

2.0W

机构

decade, inc.

学者数:

论文数: 1

被引数: 0

The Align Foundation

学者数:

论文数: 1

被引数: 0

doe agile biofoundry

学者数:

论文数: 6

被引数: 0

university of california

学者数:

1.9W

论文数: 8.0K

被引数: 10

Stanford University

学者数:

9.6W

论文数: 8.2W

被引数: 17.0W

University of Ljubljana

学者数:

1.5W

论文数: 1.3W

被引数: 1.7W

University of Edinburgh

学者数:

5.2W

论文数: 4.6W

被引数: 71

myria biosciences ag

学者数:

论文数: 2

被引数: 0

查看更多机构

引用论文

Codon influence on protein expression in E. coli correlates with mRNA levels 密码子对大肠杆菌蛋白质表达的影响与mRNA水平相关

Nature

IF0

2016-01-13

OAAI

Grégory Boël; Reka Letso; Helen Neely; W. Nicholson Price; Kam-Ho Wong; Min Su; Jon D. Luff; Mayank Valecha; John K. Everett; Thomas B. Acton; Rong Xiao; Gaetano T. Montelione; Daniel P. Aalberts; John F. Hunt

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

Experimental Biology and Medicine

IF0

2021-08-23

OAAI

Caio Coutinho de Souza; Jander Matos Guimarães; Soraya dos Santos Pereira; Luis André Morais Mariúba

Engineered peptide barcodes for in-depth analyses of binding protein libraries

NATURE METHODS

IF32.1

2019-04-22

OAAI

Egloff, Pascal; Zimmermann, Iwan; Arnold, Fabian M.; Hutter, Cedric A. J.; Morger, Damien; Opitz, Lennart; Poveda, Lucy; Keserue, Hans-Anton; Panse, Christian; Roschitzki, Bernd; Seeger, Markus A.

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Journal of Cellular Physiology

IF0

2020-02-14

OAAI

Mohsen Karbalaei; Seyed A. Rezaee; Hadi Farsiani

Mapping the energetic and allosteric landscapes of protein binding domains

Nature

IF0

2022-04-06

PREAI

Andre J. Faure; Júlia Domingo; Jörn M. Schmiedel; Cristina Hidalgo-Carcedo; Guillaume Diss; Ben Lehner

Continuous directed evolution of proteins with improved soluble expression

NATURE CHEMICAL BIOLOGY

IF13.7

2018-08-20

102

OAAI

Wang, Tina; Badran, Ahmed H.; Huang, Tony P.; Liu, David R.

Recent Advances in Function-based Metagenomic Screening

GENOMICS PROTEOMICS & BIOINFORMATICS

IF7.9

2018-12-29

108

OAAI

Ngara, Tanyaradzwa Rodgers; Zhang, Houjin

A foundation model of transcription across human cell types 人类细胞类型间的转录基础模型

NATURE

IF48.5

2025-01-08

OAAI

Fu, Xi; Mo, Shentong; Buendia, Alejandro; Laurent, Anouchka P.; Shao, Anqi; Alvarez-Torres, Maria del Mar; Yu, Tianji; Tan, Jimin; Sagatelian, Romella; Ferrando, Adolfo A.; Ciccia, Alberto; Lan, Yanyan; Owens, David M.; Palomero, Teresa; Xing, Eric P.; Rabadan, Raul

Protein aggregation in bacteria

clinical microbiology reviews

IF12.3

2019-10-21

135

OAAI

Schramm, Frederic D.; Schroeder, Kristen; Jonas, Kristina

Learned protein embeddings for machine learning

Bioinformatics

IF0

2018-06-22

OAAI

Kevin K Yang; Zachary Wu; Claire N Bedbrook; Frances H Arnold

查看更多内容