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Computational hyperspectral light-sheet microscopy
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DOI：10.1364/OE.442043.png)
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We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens. To acquire the spatial dimension orthogonal to the slit of the spectrometer, we propose to illuminate the specimen with a sequence of structured light patterns and to solve the image reconstruction problem. Beam shaping is obtained simply using a digital micromirror device in conjunction with a traditional selective plane illumination microscopy setup. We demonstrate the feasibility of this method and report the first results in vivo in hydra specimens labeled using two fluorophores. (C) 2022 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Keyword:
FLUORESCENCE MICROSCOPY
CELL
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
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引用论文
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.png)
NATURE COMMUNICATIONS
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NATURE COMMUNICATIONSIF15.7
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