.png){kind=icon}
返回
Computational single-objective scanning light sheet (cSOLS)
.png){kind=icon}
OA
AI
DOI：10.1063/5.0091615.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Single-objective scanning light sheet (SOLS) imaging has fueled major advances in volumetric bioimaging because it supports low phototoxic, high-resolution imaging over an extended period. The remote imaging unit in the SOLS does not use a conventional epifluorescence image detection scheme (a single tube lens). In this paper, we propose a technique called the computational SOLS (cSOLS) that achieves light sheet imaging without the remote imaging unit. Using a single microlens array after the tube lens (lightfield imaging), the cSOLS is immediately compatible with conventional epifluorescence detection. The core of cSOLS is a Fast Optical Ray (FOR) model. FOR generates 3D imaging volume (40 x 40 x 14 mu m(3)) using 2D lightfield images taken under SOLS illumination within 0.5 s on a standard central processing unit (CPU) without multicore parallel processing. In comparison with traditional lightfield retrieval approaches, FOR reassigns fluorescence photons and removes out-of-focus light to improve optical sectioning by a factor of 2, thereby achieving a spatial resolution of 1.59 x 1.92 x 1.39 mu m(3). cSOLS with FOR can be tuned over a range of oblique illumination angles and directions and, therefore, paves the way for next-generation SOLS imaging. cSOLS marks an important and exciting development of SOLS imaging with computational imaging capabilities. (C) 2022 Author(s).All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY)license (http://creativecommons.org/licenses/by/4.0/).
Keyword:
MICROSCOPY
FIELD
DYNAMICS
期刊
IF:
5.3
论文数:
1.5K
被引数:
5.5K
机构
引用论文
分享收藏Rapid image deconvolution and multiview fusion for optical microscopy用于光学显微镜的快速图像去卷积和多视图融合.png)
NATURE BIOTECHNOLOGY
OAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolutionNATURE METHODSOAAI
NATURE METHODSIF32.1
OAAI分享收藏Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0NATURE METHODSOAAI
NATURE METHODSIF32.1
OAAI分享收藏分享收藏Oblique-plane single-molecule localization microscopy for tissues and small intact animalsNATURE METHODS
NATURE METHODSIF32.1
PREAI
分享收藏
查看更多内容