Directed evolution approaches for optogenetic tool development

doi:10.1042/BST20210700

2021-11-16

PRE

Jang, Jaewan

G. Andrew Woolley *

DOI：10.1042/BST20210700

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摘要

En 中文

Photoswitchable proteins enable specific molecular events occurring in complex biological settings to be probed in a rapid and reversible fashion. Recent progress in the development of photoswitchable proteins as components of optogenetic tools has been greatly facilitated by directed evolution approaches in vitro, in bacteria, or in yeast. We review these developments and suggest future directions for this rapidly advancing field.

Keyword:

FLUORESCENT PROTEINS

TRANSCRIPTION FACTOR

OPTICAL CONTROL

LIGHT

SYSTEM

PHOTORECEPTORS

LOV

CYANOBACTERIOCHROMES

LOCALIZATION

RESOLUTION

期刊

Biochemical Society Transactions

IF:

4.3

论文数:

7.8K

被引数:

1.4W

机构

university of toronto

学者数:

14.8W

论文数: 12.0W

被引数: 165

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