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Drying process strongly affects probiotics viability and functionalities

delete2015-11-01
delete91
PRE
AI
C
Cyril Iaconelli
N
Noura Kechaou
F
Florian Chain
L
Luis G. Bermúdez‐Humarán
P
Philippe Langella
P
Patrick Gervais
L
Laurent Beney *
DOI:10.1016/j.jbiotec.2015.08.022delete
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摘要

摘要

En 中文
Probiotic formulations are widely used and are proposed to have a variety of beneficial effects, depending on the probiotic strains present in the product. The impact of drying processes on the viability of probiotics is well documented. However, the impact of these processes on probiotics functionality remains unclear. In this work, we investigated variations in seven different bacterial markers after various desiccation processes. Markers were composed of four different viability evaluation (combining two growth abilities and two cytometric measurements) and in three in vitro functionalities: stimulation of IL-10 and IL-12 production by PBMCs (immunomodulation) and bacterial adhesion to hexadecane. We measured the impact of three drying processes (air-drying, freeze-drying and spray-drying), without the use of protective agents, on three types of probiotic bacteria: Bifidobacterium bifidum, Lactobacillus plantarum and Lactobacillus zeae. Our results show that the bacteria respond differently to the three different drying processes, in terms of viability and functionality. Drying methods produce important variations in bacterial immunomodulation and hydrophobicity, which are Correlated. We also show that adherence can be-stimulated (air-drying) or inhibited (spray-drying) by drying processes. Results of a multivariate analysis show no direct correlation between bacterial survival and functionality, but do show a correlation between probiotic responses to desiccation-rewetting and the process used to dry the bacteria. (C) 2015 Elsevier B.V. All rights reserved.
Keyword:
Probiotics
Drying
Functionality
Viability
Process
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期刊

Journal of Biotechnology 封面图
Journal of Biotechnology
IF:
3.9
论文数:
1.5W
被引数:
1.5W

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A
agrosup dijon
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Universite de Bourgogne
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Institut Agro
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