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Focal modulation microscopy
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DOI：10.1364/OE.16.018764.png)
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We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated. (c) 2008 Optical Society of America
Keyword:
OPTICAL COHERENCE TOMOGRAPHY
2-PHOTON MICROSCOPY
BRAIN-TISSUE
RESOLUTION
DEPTH
LIGHT
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
机构
引用论文
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