High-throughput functional genomics using CRISPR-Cas9

Ophir Shalem

doi:10.1038/nrg3899

2015-04-09

1.1K

Ophir Shalem *

Neville E. Sanjana

Feng Zhang (Feng Zhang)

DOI：10.1038/nrg3899

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摘要

En 中文

Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.

Keyword:

SEQUENCE-SPECIFIC CONTROL

LENTIVIRAL RNAI LIBRARY

CANCER GENE DISCOVERY

DOUBLE-STRANDED-RNA

HUMAN-CELLS

IN-VIVO

SHRNA LIBRARIES

TARGET DNA

CAENORHABDITIS-ELEGANS

NUCLEASE SPECIFICITY

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期刊

Nature Reviews Genetics

IF:

论文数:

4.0K

被引数:

4.3W

机构

Harvard University

学者数:

26.5W

论文数: 22.0W

被引数: 28.7W

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