Imaging mass cytometry

Lasse Meyer

doi:10.1038/s43586-026-00518-y

2026-09-03

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DOI：10.1038/s43586-026-00518-y

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摘要

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成像质谱细胞术（IMC）是一种高度多重化的组织成像技术，能够在亚细胞分辨率下同时原位检测多达45种标志物。通过结合金属同位素偶联抗体等探针、激光烧蚀和时间飞行质谱技术，IMC生成具有空间分辨的高维单细胞数据。自2014年问世以来，IMC已成为空间生物学中广泛采用的平台。研究人员已利用IMC推动对癌症、自身免疫性疾病和感染性疾病的分子水平理解，该平台也日益应用于转化医学和临床领域。在本综述中，我们提供了IMC实验工作流程的全面指南，涵盖抗体偶联与面板设计、样品制备、数据采集和质量控制。我们详细介绍了计算分析流程，包括图像预处理、分割、特征提取以及单细胞和空间分析。重点突出了其在生物和临床研究中的关键应用。我们讨论了当前局限性及优化策略。最后，概述了未来发展方向，包括IMC基础模型、多模态整合以及临床实施路径。本综述旨在为IMC新手和专家提供详细见解和实用指导，以充分发掘IMC的潜力。成像质谱细胞术是一种高度多重化的组织成像技术，可在亚细胞水平同时原位检测多达45种标志物。在本综述中，Meyer等人提供了成像质谱细胞术实验工作流程的全面指南。

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Nature Reviews Methods Primers

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9.3K

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university of zurich

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5.1W

论文数: 4.0W

被引数: 65

navignostics ag

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论文数: 1

被引数: 0

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