.png){kind=icon}
返回
Metal ions shape α-synuclein
.png){kind=icon}
OA
AI
DOI：10.1038/s41598-020-73207-9.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
alpha-Synuclein is an intrinsically disordered protein that can self-aggregate and plays a major role in Parkinson's disease (PD). Elevated levels of certain metal ions are found in protein aggregates in neurons of people suffering from PD, and environmental exposure has also been linked with neurodegeneration. Importantly, cellular interactions with metal ions, particularly Ca2+, have recently been reported as key for alpha-synuclein's physiological function at the pre-synapse. Here we study effects of metal ion interaction with alpha-synuclein at the molecular level, observing changes in the conformational behaviour of monomers, with a possible link to aggregation pathways and toxicity. Using native nano-electrospray ionisation ion mobility-mass spectrometry (nESI-IM-MS), we characterize the heterogeneous interactions of alkali, alkaline earth, transition and other metal ions and their global structural effects on alpha-synuclein. Different binding stoichiometries found upon titration with metal ions correlate with their specific binding affinity and capacity. Subtle conformational effects seen for singly charged metals differ profoundly from binding of multiply charged ions, often leading to overall compaction of the protein depending on the preferred binding sites. This study illustrates specific effects of metal coordination, and the associated electrostatic charge patterns, on the complex structural space of the intrinsically disordered protein alpha-synuclein.
Keyword:
PARKINSONS-DISEASE
ALZHEIMERS-DISEASE
AMYLOID FIBRILS
BETA-SYNUCLEIN
BINDING-SITES
PROTEIN
AGGREGATION
CATIONS
COORDINATION
FIBRILLATION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.9
论文数:
28.0W
被引数:
83.5W
机构
引用论文
Effects of drought and nitrogen addition on growth and leaf physiology of Pinus massoniana seedlings
PREAI
分享收藏
Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein.png)
ANALYTICAL CHEMISTRYOAAI
ANALYTICAL CHEMISTRYIF6.7
OAAI分享收藏Metal concentrations and distributions in the human olfactory bulb in Parkinson's diseaseSCIENTIFIC REPORTSOAAI
SCIENTIFIC REPORTSIF3.9
OAAI分享收藏
查看更多内容