Millstone: software for multiplex microbial genome analysis and engineering

doi:10.1186/s13059-017-1223-1

2017-05-25

Michael G. Napolitano

Kevin Y. Chen

Changping Chen

George M. Church *

DOI：10.1186/s13059-017-1223-1

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摘要

En 中文

Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform that automates genotype comparison and visualization for projects with up to hundreds of genomic samples. To enable iterative genome engineering, Millstone allows users to design oligonucleotide libraries and create successive versions of reference genomes. Millstone is open source and easily deployable to a cloud platform, local cluster, or desktop, making it a scalable solution for any lab.

Keyword:

Synthetic biology

Bioinformatics

Synthetic genomics

Genome engineering

Microbial evolution

Whole-genome sequencing

Laboratory evolution

Experimental evolution

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Genome Biology

IF:

9.4

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6.4K

被引数:

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Harvard University

学者数:

26.5W

论文数: 22.0W

被引数: 28.7W

Harvard Medical School

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6.5W

论文数: 4.8W

被引数: 91

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