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Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array
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DOI：10.1038/nbt.3737.png)
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摘要
En 中文
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpfl to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
Keyword:
HUMAN-CELLS
RNA
SYSTEM
VECTOR
CPF1
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期刊
IF:
41.7
论文数:
1.2W
被引数:
10.1W
机构
引用论文
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