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Nanoscale resolution in GFP-based microscopy
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DOI：10.1038/NMETH922.png)
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We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
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FLUORESCENT PROTEIN
STIMULATED-EMISSION
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